Discontinuous Epitopes of Hepatitis B Surface Antigen Derived from a Filamentous Phage Peptide Library
β Scribed by Y.-C. Jack Chen, Kathy Delbrook, Chris Dealwis, Larry Mimms, Isa K. Mushahwar and Woldek Mandecki
- Book ID
- 123639497
- Publisher
- National Academy of Sciences
- Year
- 1996
- Tongue
- English
- Weight
- 936 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/38454
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π SIMILAR VOLUMES
M13 phages that display random disulfide constrained heptapeptides on their gpIII proteins were used to select for high affinity ligands to hepatitis B core antigen (HBcAg). Phages bearing the amino acid sequences C-WSFFSNI-C and C-WPFWGPW-C were isolated, and a binding assay in solution showed that
## Abstract The epitope recognized by the monoclonal antibody MA18/7, specific for the PreS1βdomain of the hepatitis B virus surface antigen, has been defined precisely by means of a library of fusionphage carrying random hexapeptides on the tip of filamentous phage fd particles. Phage, isolated af