Directional Coupling of Synthetic Peptides to Poly-L-Lysine and Applications to the ELISA

✍ Scribed by C.V. Gegg; M.E. Etzler

• Book ID: 102965241
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 503 KB
• Volume: 210
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1200

✦ Synopsis

Synthetic peptides are increasingly being used as antigens to generate highly specific antisera. Screening the antipeptide sera by enzyme-linked immunosorbent assay (ELISA) can suffer from carrier crossreactivity or lack sensitivity due to poor adsorption or presentation of the peptides. In this work we describe a procedure utilizing a heterobifunctional crosslinker to effect the directional coupling of synthetic peptides to poly-Llysine preadsorbed to microtiter plates. Plates prepared by this method conferred precision, sensitivity, and specificity to an ELISA for antipeptide antisera. In a competitive ELISA format this method permitted detection of specific peptides to (3.7 \times 10^{-10} \mathrm{M}) and provided an assay sensitive to protein structure in solution. co 1993 Academic Press, Inc.