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Direction of DNA sequences within chromatids determined using strand-specific FISH

โœ Scribed by Julianne Meyne; Edwin H. Goodwin

Book ID
104899123
Publisher
Springer
Year
1995
Tongue
English
Weight
393 KB
Volume
3
Category
Article
ISSN
0967-3849

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A simple procedure for the precise assembly of functional DNA sequences from overlapping fragments is described. The fragments to be joined are cloned in tandem in the proper relative orientation into a vector from which single-stranded DNA copies can be obtained. Single-stranded DNA is cut by a res