Directed Evolution of Cyclohexanone Monooxygenases: Enantioselective Biocatalysts for the Oxidation of Prochiral Thioethers

✍ Scribed by Manfred T. Reetz; Franck Daligault; Birgit Brunner; Heike Hinrichs; Alfred Deege

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 108 KB
• Volume: 43
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/anie.200460311

✦ Synopsis

In the preceding communication in this issue we reported that the methods of directed evolution can be used to evolve enantioselective mutants of the cyclohexanone monooxygenase (CHMO) from Acinetobacter sp. NCIMB 9871 (EC 1.14.13.22) [1] as catalysts in the Baeyer-Villiger (BV) reaction of 4-hydroxycyclohexanone and other 4-substituted cyclohexanone derivatives. [2] Since the isolated form of this flavindependent enzyme requires co-factor regeneration, we preferred to use whole cells, dioxygen from air serving as the oxidant. In view of the well-known fact that CHMOs can also