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Directed Evolution of an Error-Prone T7 DNA Polymerase that Attenuates Viral Replication

✍ Scribed by Sandra Söte; Stefan Kleine; Marina Schlicke; Dr. Susanne Brakmann


Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
385 KB
Volume
12
Category
Article
ISSN
1439-4227

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An Error-Prone T7 RNA Polymerase Mutant
✍ Susanne Brakmann; Sandra Grzeszik 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 210 KB

Viruses replicate their genomes at exceptionally high mutation rates. Their offspring evolve rapidly and therefore, are able to evade common immunological and chemical antiviral agents. In parallel, virus genomes cannot tolerate a further increase in mutation rate: Experimental evidence exists that