Direct yolk sac volume manipulation of zebrafish embryos and the relationship between offspring size and yolk sac volume

✍ Scribed by D. Jardine; M. K. Litvak

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 114 KB
• Volume: 63
• Category: Article
• ISSN: 0022-1112
• DOI: 10.1046/j.1095-8649.2003.00161.x

✦ Synopsis

Through direct manipulation of yolk sac volume (V~y~), in the zebrafish Danio rerio, V~y~ and offspring size was positively correlated and the relationship was independent of geographical location, parental size or age and, most importantly, parental genetic factors. Larval survivorship, under a non‐feeding regime and up until hatch, was not significantly affected by the manipulation. Decreased V~y~ significantly decreased maximum standard length (L~S~), maximum body surface area (A~B~), time to yolk sac absorption, L~S~ and A~B~ at yolk sac absorption, and the L~S~ and A~B~ at starvation. The methodology of V~y~ adjustment will be useful for the studies of the interaction between early life‐history traits and offspring size, egg quality variables and early vertebrate development.