Direct visualization of collagens and procollagens in polyacrylamide gels

✍ Scribed by Paulette J. McCormick; Srinivasan Chandrasekhar; Albert J.T. Millis

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 770 KB
• Volume: 97
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90086-1

✦ Synopsis

This report describes a procedure that results in the rapid visual identification of collagens and procollagens in polyacrylamide gels. The technique results in a pink stain for collagenous proteins and a blue stain for all other proteins. The color difference has been evaluated spectrophotometrically.

The absorbance maxima for collagen-Coomassie blue R250 complexes in gels is 520-535 nm, and the maxima for all other protein-Coomassie blue R250 complexes that we tested is 550-560 nm. This technique will facilitate the identification of collagenous proteins in complex mixtures of proteins derived from cell membranes, whole cell extracts, conditioned media, and extracellular matrices. We use the technique to detect procollagens in human diploid fibroblast conditioned media. The technique is simple, relatively rapid, has utility for proteins extracted by a variety of methods, and is applicable to all polyacrylamide gel systems in general use.