Direct vapor generation Fourier transform infrared spectrometric determination of ethanol in blood

✍ Scribed by Amparo Pérez-Ponce; Salvador Garrigues; Miguel de la Guardia

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 589 KB
• Volume: 336
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(96)00386-8

✦ Synopsis

A new procedure is proposed for a direct determination of ethanol in plasma and whole blood. The method is based on the injection of a discrete sample volume of 10~1 into an electrically heated Pyrex glass reactor in which, at a temperature of 90°C the ethanol is volatilized and introduced by means of a N2 carrier flow inside a long-path infrared gas cell and the corresponding flow analysis recording registered as a function of time. The measurement of the area of the flow injection recording, obtained from the absorbance of the transient signal in the range 1150-950 cm-' , allows the direct quantification of ethanol upto 2g 1-l. with a limit of detection of O.O2Ogl-' and coefficient of variation between 0.3 and 1.9% for three replicate analyses of the same sample. The analysis frequency of the method is 40 hh', and it can be applied to a single drop of finger blood.