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Direct spectrophotometric determination of proteins immobilized on bead cellulose and dissolved in cadoxene

✍ Scribed by Peter Gemeiner; Mikuláš Pašteka


Book ID
112890918
Publisher
Springer-Verlag
Year
1983
Tongue
English
Weight
568 KB
Volume
8
Category
Article
ISSN
0273-2289

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Kinetic comparisons have been made between a randomly immobilized and a site-specifically immobilized subtilisin BPNЈ on microfiltration membranes of varying hydrophilicities in both aqueous and organic media. Site-directed mutagenesis was employed to introduce a single cysteine into the amino acid