A total extract dot blot hybridization p
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Andrew Grimes; Harry J. McArdle; Julian F.B. Mercer
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Article
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1988
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Elsevier Science
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English
⚖ 596 KB
A simple method for the estimation of specific mRNA concentrations in small tissue samples (as little as 1 mg) or cultured cells (lower limit 10(5) cells) is described. Guanidine hydrochloride extracts of whole cells or tissues are applied directly onto nitrocellulose and hybridized with the appropr