✦ LIBER ✦
Direct serotyping of porcine rotaviruses using VP7-specific monoclonal antibodies by an enzyme immunoassay
✍ Scribed by H. S. Nagesha; I. H. Holmes
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 595 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Employing a serotyping EIA test using Mabs both cell culture adapted and faecal porcine rotaviruses were classified into serotypes G3, G3/5, G4, and G5. The MAbs have confirmed and extended the serotyping results obtained using polyclonal antisera. These MAbs are therefore potential reagents for serotyping of porcine ro‐taviruses. Using subgroup specific MAbs sero‐types G3, G3/5, and G5 were found to contain subgroup I antigens while G4 rotaviruses contained either subgroup II or subgroup I antigens.