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Direct selection of DNA inserts in plasmid gene of kanamycin-resistance

โœ Scribed by Slutsky, Alexander M. ;Rabinovich, Pyotre M. ;Yakubov, Leonid Z. ;Sineokaya, Irene V. ;Stepanov, Anatoly I. ;Gordeyev, Vyacheslav K.

Book ID
104770559
Publisher
Springer
Year
1980
Tongue
English
Weight
139 KB
Volume
180
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

In this paper we propose a method of direct selection for hybrid DNA molecules in paromomycin-dependent E. coli cells.

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Transposition of DNA inserted into delet
Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element
โœ Meyer, Richard ;Boch, Gary ;Shapiro, James ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 716 KB

Tn5-trp hybrid transposons have been constructed by insertion of a trpPOED Hind III fragment into an in vivo Tn5 internal deletion mutant or by substitution of trp for the internal Tn5 Hind III fragment. These hybrids are called, respectively, Tn409 and Tn410. Both Tn409 and Tn410 will transpose int