✦ LIBER ✦
Direct recombinogenic effect of dimethylnitrosamine (DMN) on zygotes ofChlamydomonas reinhardtii
✍ Scribed by Annekathrin Preidel; Oskar Schimmer
- Publisher
- Springer-Verlag
- Year
- 1986
- Tongue
- English
- Weight
- 277 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
✦ Synopsis
We developed a test system with mutant strains of Chlamydomonas reinhardtii requiring arginine. Here, the meiotic recombination between two arginine loci served as an indicator of genotoxicity. After crossing, zygotes were treated with the alkylating agent dimethyl-nitrosamine (DMN) for 30 min. A dose dependent increase of wild type recombinants was found for DMN concentrations ranging from 68 microM to 680 microM. Like in cells of some higher plants, the agent was active in Chlamydomonas zygotes without any exogenous metabolic activation.