Direct quantitation of HIV by flow cytometry using branched DNA signal amplification
β Scribed by M Van Cleve; N Ostrerova; K Tietgen; W Cao; C Chang; M.L Collins; J Kolberg; M Urdea; K Lohman
- Book ID
- 115638257
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 194 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0890-8508
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## Abstract ## Background Complex mixtures of DNA may be found in environmental and medical samples. There is a need for techniques that can measure low concentrations of target DNAs. For a multiplexed, flow cytometric assay, we show that the signalβtoβnoise ratio for fluorescence detection may be
A procedure for amplifying and detecting nucleic acid sequences in situ using cells in suspension and flow cytometry has been developed. The process involves the use of the polymerase chain reaction (PCR) and a fluorescent in situ hybridization (FISH) protocol developed in our laboratory to detect t