Direct outgrowth of in vivo epstein-barr virus (EBV)-infected chronic lymphocytic leukemia (CLL) cells into permanent lines
✍ Scribed by Nongnit Lewin; Pierre Åman; Håkan Mellstedi; Lore Zech; George Klein
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- French
- Weight
- 444 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
In the course of our efforts to characterize the EBV-carrying occasions at an interval of 3 months. The patient's antibody cells that are responsible for direct growth or the 2-step mech-titer to EBV-determined nuclear antigen (EBNA) was 1:320, anism, based on virus release from the explanted cells and to early antigens (EA) 1:640 and to viral capsid antigens subsequent transformation of previously uninfected cells, we (VCA) 1 : 10,240. His lymphocytes were isolated by Ficollhave encountered an unusual CLL patient who carried a small isopaque gradient centrifugation ( ~b ~~~, 1977) and separated subpopulation of in vivo EBV-infected leukemia cells. These grew into EBV-carrying lines upon explantation after a rela. addition, cord-blood lymphocytes were isolated as described tively short latency period, 3-4 weeks. Cytogenetic examina-above.
tion conclusively proved the leukemic origin of the established CLL lines. They carry a ring chromosome I S and are trisomic for chromosome 12. The same changes are also found in the