โ Scribed by Emile van Handel
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No direct method appears to be available for the determination of sucrose in carbohydrate mixtures. The polarimetric, oxidimetric, and enzymic methods depend on the measurement of a difference before and after hydrolysis. They are inadequate when sucrose constitutes only a small percentage of the carbohydrate fraction. Quantitative chromatographic separation is laborious and not suitable in the presence of a large excess of other sugars. Furthermore, some disaccharides cannot easily be separated from sucrose in this way. During a study of the synthesis of sucrose in insects, a micromethod was required which accurately measured sucrose in a large excess of fructose and glucose. The proposed procedure depends on the destruction of reducing sugars with hot concentrated alkali followed by determinat'ion, using anthrone at low temperature, of the fructose moiety of sucrose.
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