Direct measurement of the transverse and longitudinal 15N chemical shift anisotropy–dipolar cross-correlation rate constants using 1H-coupled HSQC spectra

✍ Scribed by Jennifer B. Hall; David Fushman

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 149 KB
• Volume: 41
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1253

✦ Synopsis

Abstract

We describe direct methods for the measurement of the transverse and longitudinal ^15^N chemical shift anisotropy–dipolar cross‐correlation rates based on comparison of the ^15^N doublet components observed in ^1^H‐coupled ^1^H–^15^N HSQC‐type spectra. This allows the determination of the cross‐correlation rates with no need for correction factors associated with other methods. The signal overlap problem of coupled HSQC spectra is addressed by using the IPAP scheme (Ottiger M, Delaglio F, Bax A. J. Magn. Reson. 1998; 131: 373). The methods proposed here use a conventional t~1~ evolution period, which allows one to minimize the truncation artifacts observed in a constant‐time‐type experiment (Hall JB, Dayie K, Fushman D. J. Biomol. NMR 2003; 26: 181). Applications of these measurements to the B3 domain of protein G are discussed. Copyright © 2003 John Wiley & Sons, Ltd.