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Direct Measurement of Oligonucleotide Binding Stoichiometry of Gene V Protein by Mass Spectrometry

โœ Scribed by Xueheng Cheng, Amy C. Harms, Paul N. Goudreau, Thomas C. Terwilliger and Richard D. Smith

Book ID
123639941
Publisher
National Academy of Sciences
Year
1996
Tongue
English
Weight
954 KB
Volume
93
Category
Article
ISSN
0027-8424

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## Abstract The calciumโ€binding properties of parvalbumin and calmodulin were investigated by electrospray ionization mass spectrometry (ESIโ€MS). The two calcium sites of parvalbumin were found to be strongly cooperative in binding Ca^2+^ ion. Up to four calcium ions were found to bind to calmoduli