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Direct Measurement of Initiation, Elongation, Splicing and Termination Kinetics in Living Human Cells

✍ Scribed by Coulon, Antoine; Ferguson, Matthew L.; Palangat, Murali; de Turris, Valeria; Singer, Robert H.; Chow, Carson C.; Larson, Daniel R.

Book ID
122126175
Publisher
Biophysical Society
Year
2013
Tongue
English
Weight
52 KB
Volume
104
Category
Article
ISSN
0006-3495

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## Background: The Cellscan mark-S (CS-S) scanning cytometer was used for tracing enzymatic reactions in the same individual cells under various physiological conditions over periods of minutes. On-line reagent addition and changes in the experimental conditions (buffers, ions, substrates and inhib