Direct isotope determination of drugs labeled with stable isotopes of hydrogen, carbon, and nitrogen by field desorption mass spectrometry

✍ Scribed by U. Bahr; H.-R. Schulten

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: French
• Weight: 375 KB
• Volume: 18
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580180415

✦ Synopsis

Abstract

By recording the molecular ion group with repetitive magnetic scanning and accumulation of the mass spectra in a multichannel analyser field desorption mass spectrometry was used to determine the isotopic abundances of three widely used drugs in their unlabeled and stable‐isotope labeled forms. The label content and statistical distribution of ^2^H, ^13^C and ^15^N in analogs of an antitumor agent, cyclophosphamide and one of its metabolites 4‐ketocyclophosphamide and of two analgesics antipyrine and carbamazepine were investigated. Using nanogram amounts of sample the precision and accuracy of the measurements achieved were below ±1% on an absolute basis.