Direct injection of large sample volumes into capillary columns with packed column injector

✍ Scribed by Watanabe, Chuichi ;Hashimoto, Keiji

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 381 KB
• Volume: 13
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240130905

✦ Synopsis

Abstract

A direct injection method for large volume samples which avoids severe tailing of the solvent peak has been developed using a packed column injector (up to 100 μl) leading into an ordinary capillary column (0.3 mm i.d.). Modifications are made to the cooler zones of the inlet port and on the carrier gas flow control system. This injection technique is based on the effective use of phase soaking and cold trapping using a retention gap. The large volume of solvent vapor is rapidly purged out of the injector with a higher flow of carrier gas while the solutes trapped at the head of the column are subsequently analyzed with another optimum flow rate. The proposed carrier gas flow regulation system is also compared with conventional split/splitless injection methods.