Direct in situ reverse transcriptase-linked polymerase chain reaction with biotinylated primers for the detection of hepatitis C virus RNA in liver biopsies
✍ Scribed by Dominique Bettinger; Christiane Mougin; Brigitte Fouqué; Bernadette Kantelip; Jean Philippe Miguet; Michel Lab
- Book ID
- 117771565
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 318 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1386-6532
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## Abstract Detection of hepatic hepatitis C virus RNA and antigens is difficult since their expression is very low. The technique of in situ reverse transcription polymerase chain reaction (IS‐RT‐PCR) was developed for the detection and localization of HCV RNA in formalin‐fixed paraffin‐embedded l
The cellular localization of hepatitis C virus (HCV) RNA in liver tissue was studied by nonisotopic in situ hybridization using a digoxigenin-labeled cDNA probe created during a polymerase chain reaction on samples from 16 patients with chronic HCV infection. Hybridization signalswere recognized in