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Direct immunoassay of estrone by capillary electrophoresis with laser-induced fluorescence detection

โœ Scribed by Ping Su; Xin-Xiang Zhang; Wen-Bao Chang

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
110 KB
Volume
24
Category
Article
ISSN
0173-0835

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โœฆ Synopsis

Abstract

An immunoassay for estrone (E~1~) in women's serum, based on the competitive reaction between fluoresceinโ€labeled complete antigen and E~1~ with limited amount of antiโ€estrone monoclonal antibody is described. A thermally reversible hydrogel, polyโ€Nโ€isopropylacrylamide (pNIPA), was added to the buffer to improve the reproducibility. With a laserโ€induced fluorescence (LIF) detector, the capillary electrophoretic immunoassay (CEIA) can be applied to determine E~1~ at a concentration lower than 19.6ย pg/mL. The E~1~ levels in ten normal women's serum were measured at the range of 118.6โ€“222.0ย pg/mL.

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