Direct identification of human enterovirus serotypes in cerebrospinal fluid by amplification and sequencing of the VP1 region
β Scribed by E.C. McWilliam Leitch; H. Harvala; I. Robertson; I. Ubillos; K. Templeton; P. Simmonds
- Book ID
- 116631519
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 600 KB
- Volume
- 44
- Category
- Article
- ISSN
- 1386-6532
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To assess the prevalent genetic types of human parvovirus B19 strains derived from various sources and their relation to particular clinical symptoms, the genetic diversity in the VP1 unique region, which is important for the neutralizing response to human parvovirus B19, was examined by the mismatc
Three nested RT-PCR assays were developed to permit sensitive typing of enteroviruses directly from clinical samples. These assays amplified short fragments from different genomic regions codifying for three proteins: VP2, VP1, and RNA polymerase. Given that enteroviruses have a high rate of degener