𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Direct from Polyacrylamide Gel Infrared Laser Desorption/Ionization

✍ Scribed by Xu, Yichuan; Little, Mark W.; Rousell, David J.; Laboy, Jorge L.; Murray, Kermit K.

Book ID
127259011
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
89 KB
Volume
76
Category
Article
ISSN
0003-2700

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Infrared laser desorption and ionization
Infrared laser desorption and ionization of polypeptides from a polyacrylamide gel
✍ Michelle Baltz-Knorr; David R. Ermer; Kenneth E. Schriver; Richard F. Haglund Jr 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 242 KB

## Abstract We observed direct desorption and ionization of angiotensin II and bovine insulin from a frozen polyacrylamide gel without the addition of an exogenous matrix, using picosecond pulses from a tunable, mid‐infrared free‐electron laser tuned to strong absorption bands of the gel. At 5.7, 5

Infrared-matrix-assisted laser desorptio
Infrared-matrix-assisted laser desorption/ionization and infrared-laser desorption/ionization investigations of synthetic polymers
✍ Steffen Weidner; Gerhard Kühn; Jörg Friedrich 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 696 KB

The application of an IR laser working at a wavelength of 3.27 mm, for matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry and matrix-less laser desorption/ionization of different polymers, has been demonstrated. The ionization/desorption of polyethyleneglycols (PEG) and po

Matrix-assisted laser desorption/ionizat
Matrix-assisted laser desorption/ionization mass spectrometry of proteins extracted directly from sodium dodecyl sulphate-polyacrylamide gels
✍ Hanno Ehring; Signhild Strömberg; Agneta Tjernberg; Bengt Norén 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 166 KB 👁 2 views

A new strategy for the characterization of Coomassie Brilliant Blue stained SDS-PAGE separated proteins by UV-MALDI-MS is reported. The proteins are extracted directly from the polyacrylamide gel by treatment with an organic solvent mixture consisting of formic acid, acetonitrile, isopropanol and wa

Matrix-free infrared soft laser desorpti
Matrix-free infrared soft laser desorption/ionization
✍ David J. Rousell; Sucharita M. Dutta; Mark W. Little; Kermit K. Murray 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 170 KB

## Abstract Infrared soft laser desorption/ionization was performed using a 2.94 µm Er : YAG laser and a commercial reflectron time‐of‐flight mass spectrometer. The instrument was modified so that a 337 nm nitrogen laser could be used concurrently with the IR laser to interrogate samples. Matrix‐as

Laser desorption-atmospheric pressure ch
Laser desorption-atmospheric pressure chemical ionization: a novel ion source for the direct coupling of polyacrylamide gel electrophoresis to mass spectrometry
✍ Joshua J. Coon; Heather A. Steele; Philip J. Laipis; W. W. Harrison 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 161 KB

## Abstract Laser desorption‐atmospheric pressure chemical ionization‐mass spectrometry (LD‐APCI‐MS) is presented for the atmospheric pressure (AP) sampling of tryptic peptides directly from a polyacrylamide gel. In contrast to other gel sampling mass spectrometric approaches, this technique does n