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Direct fermentation of cassava starch to ethanol by mixed cultures ofEndomycopsis fibuligeraandZymomonas mobilis: Synergism and limitations

✍ Scribed by O. Vijaya Sarathi Reddy; S. C. Basappa

Publisher: Springer Netherlands
Year: 1996
Tongue: English
Weight: 241 KB
Volume: 18
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00129962

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✦ Synopsis

A mixed culture of Endomycopsis fibuligera NRRL 76 and Zymomonas mobilis ZM4 could directly and more efficiently ferment cassava starch (22.5% w/v) to ethanol (10.5% v/v) than the monocultures. The combination of culture filtrate of E.fibuligera containing amylases and Z.mobilis simultaneously saccharified and fermented the cassava starch to ethanol equally well. Giucoamylase (0.01%) added to the fermenting medium improved ethanol (13.2% v/v) production by the above mixed culture to almost the theoretical level (98%) indicating that this enzyme is a rate-limiting factor in E.fibuligera. Z. mobilis alone converted the enzymehydrolyzed starch only to almost theoretical level (98%).

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The conversion of starch from unhydrolyzed cassava flour to ethanol by a pure culture of Endomycopsis fibdigera and by a co-culture of this amylolytic yeast and the bacterium Zymmonas mobilis was studied. The best overall results were obtained using the mixed culture. After 96 h of fermentation of