✦ LIBER ✦

Direct estimation of serological H-Y antigen by flow cytometry

✍ Scribed by Marijo Kent; Stephen Wachtel; H. Tzvi Thaler

Publisher: Springer
Year: 1990
Tongue: English
Weight: 293 KB
Volume: 85
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00276339

No coin nor oath required. For personal study only.

✦ Synopsis

This study was undertaken to evaluate the efficiency of flow cytometry in the detection of the serological H-Y antigen, and to survey expression of H-Y in the normal human population. Peripheral blood leukocytes (granulocytes) were reacted with monoclonal H-Y antibody, gw-16, and with FITC-conjugated goat anti-mouse IgG, and then were scored for fluorescence in the flow cytometer. Assigned H-Y phenotype was correlated accurately with sex phenotype in 33 out of 38 women and 62 out of 65 men. Data were evaluated by exploratory data analysis (EDA).

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