Direct Enzymatic Analysis of Glycerol inHoney: a Simplified Method

✍ Scribed by Fernández-Muíño, Miguel A; Sancho, M Teresa; Muniategui, Soledad; Huidobro, José F; Sánchez, M Pilar; Simal-Lozano, Jesús

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 334 KB
• Volume: 71
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(199606)71:2<141::aid-jsfa547>3.0.co;2-n

✦ Synopsis

The glycerol content of honey has been determined using a Boehringer Mannheim enzymatic method simplified for this purpose. The solution of honey was employed directly, without clarification and without addition of NaOH solution. With these conditions, there were no observed interference effects. It is necessary to use a 1.5 ml micro-cuvette. The enzymatic determination was measured spectrophotometrically at 365 nm using the enzymes in quadruple the quantities recommended by the supplier. The method combines precision, good recovery, sensitivity, simplicity and low cost.