Direct Electron Transfer for Heme Proteins Assembled on Nanocrystalline TiO2 Film
✍ Scribed by Qingwen Li; Guoan Luo; Jun Feng
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 145 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
Nanosized TiO 2 is now an attractive biocompatiable material widely used in toothpaste and cosmetics. Due to its unique physiochemical properties and its inclination to selectively combine with some groups of biomolecules, nanosized TiO 2 has been proposed as a promising interface for the immobilization of biomolecules. In this article, heme proteins involving cytochrome c, myoglobin and hemoglobin were attempted to be assembled onto a nanocrystalline TiO 2 ®lm, their electrochemical behaviors on such interface were characterized with cyclic voltammetry. It was proved that TiO 2 ®lm could not only offer a friendly platform to assemble protein molecules, but also enhance the electron transfer process between protein molecules and the electrode. The electrode kinetic parameters of each hemoprotein on such electrode as well as the effect of scan rate on the electrochemical behavior of each protein are discussed in details.