Direct electrochemistry of horseradish peroxidase on graphene-modified electrode for electrocatalytic reduction towards H2O2

✍ Scribed by Maoguo Li; Shudong Xu; Min Tang; Lin Liu; Feng Gao; Yinling Wang

• Publisher: Elsevier Science
• Year: 2011
• Tongue: English
• Weight: 846 KB
• Volume: 56
• Category: Article
• ISSN: 0013-4686
• DOI: 10.1016/j.electacta.2010.10.034

✦ Synopsis

Graphene was synthesized by a chemical method to reduce graphite oxide and well characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), powder X-ray diffraction (PXRD) and Fourier transform infrared (FTIR) spectra. Horseradish peroxidase (HRP) immobilized on a graphene film glassy carbon electrode was found to undergo direct electron transfer and exhibited a fast electron transfer rate constant of 4.63 s -1 . The HRP-immobilized electrode was investigated by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The CV results showed that the modified electrode gave rise to well-defined peaks in phosphate buffer, corresponding to the electrochemical redox reaction between HRP-Fe(III) and HRP-Fe(II). The obtained electrode also displayed an electrocatalytic reduction behavior towards H 2 O 2 . The new H 2 O 2 sensor shows a linear range of 0.33-14.0 M (R 2 = 0.9987) with a calculated detection limit of 0.11 M (S/N = 3). Furthermore, the biosensor exhibits both good operational storage and storage stability.