Direct Electrochemistry of Hemoglobin Immobilized on Carbon-Coated Iron Nanoparticles for Amperometric Detection of Hydrogen Peroxide

✍ Scribed by Hai-Li Zhang; Xiao-Zhi Zou; Guo-Song Lai; De-Yan Han; Fang Wang

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 187 KB
• Volume: 19
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200703942

✦ Synopsis

A novel method for preparation of hydrogen peroxide biosensor was presented based on immobilization of hemoglobin (Hb) on carbon-coated iron nanoparticles (CIN). CIN was firstly dispersed in a chitosan solution and cast onto a glassy carbon electrode to form a CIN/chitosan composite film modified electrode. Hb was then immobilized onto the composite film with the cross-linking of glutaraldehyde. The immobilized Hb displayed a pair of stable and quasireversible redox peaks and excellent electrocatalytic reduction of hydrogen peroxide (H 2 O 2 ), which leading to an unmediated biosensor for H 2 O 2 . The electrocatalytic response exhibited a linear dependence on H 2 O 2 concentration in a wide range from 3.1 mM to 4.0 mM with a detection limit of 1.2 mM (S/N ¼ 3). The designed biosensor exhibited acceptable stability, long-term life and good reproducibility.