Direct Electrochemistry of Cytochrome c at a Metallothioneins Self-Assembled Gold Electrode

✍ Scribed by Qian Wang; Nanqiang Li; Yonghui Wu

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 145 KB
• Volume: 13
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/1521-4109(200102)13:2<149::aid-elan149>3.0.co;2-3

✦ Synopsis

Horse heart cytochrome c was studied by cyclic voltammetry using a gold electrode self-assembled with the metallothioneins monolayer (MTaAu) as working electrode. A pair of well-de®ned redox waves was obtained as the MT SAM-modi®ed gold electrode in a cytochrome c aqueous solution. The calculated standard rate constant (k s ) is 2.43610 73 cm s 71 . The electrode reaction is a pseudo-reversible process. The peak current and the concentration of cytochrome c have a linear relationship in the range of 2.0610 75 mol L 71 to 4.0610 74 mol L 71 . The detection limit is 2.0610 75 mol L 71 . The surface roughness factor of the Au electrode is 2.59. The MT SAM was characterized with X-ray photoelectron spectroscopy (ARXPS).