Direct Electrochemistry and Electrocatalysis of Hemoglobin in Lipid Film Incorporated with Room-Temperature Ionic Liquid

✍ Scribed by Gaiping Li; Liangwei Du; Hongjun Chen; Lixue Zhang; Erkang Wang

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 344 KB
• Volume: 20
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200804305

✦ Synopsis

Abstract

A facile phospholipid/room‐temperature ionic liquid (RTIL) composite material based on dimyristoylphosphatidylcholine (DMPC) and 1‐butyl‐3‐methylimidazolium hexafluorophosphate ([bmim]PF~6~) was exploited as a new matrix for immobilizing protein. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were adopted to characterize this composite film. Hemoglobin (Hb) was chosen as a model protein to investigate the composite system. UV‐vis absorbance spectra showed that Hb still maintained its heme crevice integrity in this composite film. By virtue of the Hb/DMPC/[bmim]PF~6~ composite film‐modified glassy carbon electrode (GCE), a pair of well‐defined redox peaks of Hb was obtained through the direct electron transfer between protein and underlying GCE. Moreover, the reduction of O~2~ and H~2~O~2~ at the Hb/DMPC/[bmim]PF~6~ composite film‐modified GCE was dramatically enhanced.