Direct effect of phosphorus on PTH secretion from whole rat parathyroid glands in vitro

✍ Scribed by Yolanda Almaden; Antonio Canalejo; Alexis Hernandez; Evaristo Ballesteros; Socorro Garcia-Navarro; Armando Torres; Mariano Rodriguez; Mariano Rodriguez

• Publisher: American Society for Bone and Mineral Research
• Year: 2009
• Tongue: English
• Weight: 530 KB
• Volume: 11
• Category: Article
• ISSN: 0884-0431
• DOI: 10.1002/jbmr.5650110714

✦ Synopsis

Phosphorus retention is an important factor in the development of hyperparathyroidism secondary to renal failure. In vivo manipulation of phosphorus is associated with changes in serum calcium and calcitriol levels which in turn can modify parathyroid hormone synthesis and secretion. The present in vitro study evaluates whether high extracellular phosphorus has a direct effect on parathyroid hormone secretion. Fresh rat parathyroid glands were incubated in a media with phosphorus concentrations of 1,2,3, and 4 mM and subsequently exposed to calcium levels ranging from 0.4 to 1.35 mM. In 1.25 mM calcium, the parathyroid hormone secretion rate was similar in 1 and 2 mM phosphorus; however, a phosphorus concentration of 3 and 4 mM produced a 3and 4-fold increase in the parathyroid hormone secretion, respectively, as compared with 1 mM phosphorus. While in 1 or 2 mM phosphorus an increase in calcium from 0.6 to 1.35 mM reduced parathyroid hormone secretion to 3796, in 4 mM phosphorus the same increase in calcium only inhibited parathyroid hormone secretion to 75%. Furthermore, the addition of arachidonic acid 20 pM, a substrate for inhibitory intracellular signal pathway, to the 4 mM phosphorus-1.35 mM calcium incubation media reduced the parathyroid hormone secretion to 34.5% (p < 0.05).

In conclusion, our results indicate that in vitro, high phosphorus directly increases parathyroid hormone secretion.