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Direct diagnosis ofMycobacterium tuberculosisin blood samples of HIV infected patients by polymerase chain reaction

โœ Scribed by Senthilkumar Kamatchiammal; Dhashinamoorthy Saravanakumar; Nagalingeswaran Kumarasamy; Sunithi Solomon; Manjula Sritharan; Venkataraman Sritharan


Publisher
Association of Clinical Biochemistry of India
Year
2000
Tongue
English
Weight
726 KB
Volume
15
Category
Article
ISSN
0970-1915

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The clinical performance of a modified polymerase chain reaction (PCR) testing algorithm was evaluated for confirming the presence of HIV-1 proviral DNA in peripheral blood mononuclear cells. A whole cell lysate, rather than phenol-purified DNA, was used for PCR amplification, under systematically o