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Direct determination of ultrathin section thickness using latex particles

✍ Scribed by Jésior, Jean-Claude

Publisher
Wiley (John Wiley & Sons)
Year
1985
Tongue
English
Weight
383 KB
Volume
2
Category
Article
ISSN
0741-0581

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✦ Synopsis

A method is proposed to determine ultrathin section thickness by means of noncentral sections obtained from latex spheres. The resulting spherical segments provide the parameters necessary to calculate very simply the section thickness without further treatment. The method is illustrated by an example using the grid-sectioning technique and could be generalized to classical microtomy.

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