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Direct determination of the growth rate of Drosophila imaginal discs

✍ Scribed by Martin, Presley F.


Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
408 KB
Volume
222
Category
Article
ISSN
0022-104X

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✦ Synopsis


Abstract

A simple technique is described which allows the number of cells in the imaginal discs of Drosophila melanogaster to be determined directly, by dissociation of the tissue into single cells. This technique was used to determine the growth rates for the eye‐antenna, wing, leg, and haltere discs during the second and third larval instars. The results obtained for the number of cells present at particular stages and for the growth rates during this period are similar to those determined previously using either indirect or more tedious procedures.


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