Direct determination of sodium, potassium, magnesium, and calcium ions in human saliva by ion chromatography using a taurine-conjugated bile salt micelle-coated stationary phase
✍ Scribed by Wenzhi Hu; Hiroki Haraguchi
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 505 KB
- Volume
- 289
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
Ion chromatography using a taurine-conjugated bile salt micelle-coated stationary phase has been investigated for the direct determination of common metal ions in biological samples. Taurlne-conjugated bile salt micelles are reversed aggregates, where the negative charges of sulphonate form the inside of the micelle. The sulphonate charges are acting as the ion-exchange sites for the separation of cations, while the cavity of the helical micelle also acts as a size-exclusion site for the rapid elution of large organic compounds. The combination of ion-exchange and size-exclusion functions provide a new model for the direct determination of inorganic species in biological fluids using ion chromatography. The present system was successfully used for the direct determination of sodium, potassium, magnesium and calcium ions in human's mixed saliva.