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Direct Detection of Crosslinks of Collagen and Elastin in the Hydrolysates of Human Yellow Ligament Using Single-Column High Performance Liquid Chromatography

โœ Scribed by Jin Ran Chen; Masaaki Takahashi; Kazuhiro Kushida; Motohiro Suzuki; Kazutaka Suzuki; Kentaro Horiuchi; Akira Nagano

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
98 KB
Volume
278
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Collagen and elastin are recognized as two major connective tissue proteins of human yellow ligament. In both collagen and elastin there are many kinds of intra-or intermolecular crosslinks. Pyridinoline (Pyr) and deoxypyridinoline (Dpyr) are mature crosslinks which maintain the structure of the collagen fibril. Desmosine (Des) and isodesmosine (Isodes) represent the major crosslinking components of elastin. Pentosidine (Pen), which is a senescent crosslink and one of the advanced glycation end products, accumulates with age in tissue proteins including collagen. We developed a direct and one-injection HPLC method to measure Pyr, Dpyr, Des, Isodes, and Pen in the hydrolysate of human yellow ligament. This method used one column and two detectors. Recovery rates of Pyr,

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