𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Direct Cryogenic-Temperature Transmission Electron Microscopy Imaging of Phospholipid Aggregates in Soybean Oil

✍ Scribed by Dganit Danino; Rajan Gupta; Jagannadh Satyavolu; Yeshayahu Talmon

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
751 KB
Volume
249
Category
Article
ISSN
0021-9797

No coin nor oath required. For personal study only.

✦ Synopsis

Cryogenic-temperature transmission electron microscopy (cryo-TEM) was fine-tuned to make it applicable to the study of a natural oil-based system. While liquid ethane, the cryogen of choice for specimen preparation, was found to dissolve the oil, we observed that the triglycerides found in many natural oils serve as cryoprotectants that allow vitrification of such oil systems in liquid nitrogen, a cryogen that provides relatively low cooling rates. Using the modified technique combined with digital imaging by a cooled CCD camera, we were able to directly visualize inverse micellar and liquid-crystalline aggregates formed in the soybean oil/soybean phospholipids/water/hexane system, which is commercially important in soybean oil processing. The method developed here should be widely applicable to other organic solvent-based systems.

πŸ“œ SIMILAR VOLUMES

Microstructural evolution of lipid aggre
Microstructural evolution of lipid aggregates in nucleating model and human biles visualized by cryogenic transmission electron microscopy
✍ Fred M. Konikoff; Dganit Danino; Daphna Weihs; Moshe Rubin; Yeshayahu Talmon πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 625 KB

Obtaining reliable information on the physical state and ultrastructure of bile is difficult because of its mixed aqueous-lipid composition and thermodynamic metastability. We have used time-lapse cryogenic transmission electron microscopy (cryo-TEM) combined with video-enhanced light microscopy (VE

A New View on Chemistry of Solids in Sol
A New View on Chemistry of Solids in Solution-Cryo Energy-Filtered Transmission Electron Microscopy (Cryo-EFTEM) Imaging of Aggregating Palladium Colloids in Vitreous Ice
✍ Jan-Olov Bovin; Torsten Huber; Olivier Balmes; Jan-Olle Malm; Gunnel Karlsson πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 105 KB πŸ‘ 1 views

It is shown that by using cryo-transmission electron microscopy (cryo-TEM) it is possible to image the aggregation behaviour of nanoparticles while they are still in solution. This technique has allowed the study of the arrangement of colloidal palladium particles in solution by preparing the specim