Direct connection of high-speed liquid chromatograph (equipped with gel permeation column) to atomic absorption spectrophotometer for metalloprotein analysis: Metallothionein
✍ Scribed by Kazuo T. Suzuki
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 310 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A high-speed liquid chromatograph equipped with a gel permeation column (Toyo Soda TSK GEL SW 3000) was directly connected to an atomic absorption spectrophotometer for rapid analysis of metalloproteins and applied for analysis of metallothionein. Although the column used in the present study exhibits a nonselective adsorption for metals attached to biopolymers with low stability constants, the analytical system was shown to be practically useful, especially for metalloproteins with high stability constants. Metallothioneins were separated into two isometallothioneins on the column, indicating that the column has both gel chromatographic and ion-exchange chromatographic properties.