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Direct Automated Sequencing of Single λ-Phage Plaques by Exponential Amplification Sequencing

✍ Scribed by David M. Hwang; Ruo-Xiang Wang; Choong-Chin Liew


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
64 KB
Volume
231
Category
Article
ISSN
0003-2697

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✦ Synopsis


Recently, we explored the possibility of sequencing Grant CA53617. We thank M. Risin, H. Saya, H. Takeshema, and inserts directly from phage plaques. While linear am-P. Lee for their help.

plification sequencing of single l plaques had been described using manual sequencing techniques (5), this REFERENCES approach has not been successfully generalized to the 1. Smith, D. B., and Johnson, K. S. (1988) Gene 67, 31-40. automated sequencing technologies used in most large-2.