Direct atomization atomic absorption spectrometry for intact biological samples: Application to trace metal analysis in silkworm eggs and their hatched worms
✍ Scribed by Kitao Fujiwara; Yoshio Umezawa; Yu-ichi Numata; Keiichiro Fuwa; Shizuo Fujiwara
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 541 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Highly sensitive carbon rod atomic absorption for trace elements was observed by direct atomization of samples with no pretreatment for intact silkworm eggs, their hatched worms, or National Bureau of Standards biological samples (orchard leaves and bovine liver). Crucial temperatures for drying, ashing, and atomization processes were found to obtain accurate results. Results were consistent with those of activation analyses and of ordinary atomic absorption with wet ashing. The precision and dynamic range of this method were evaluated. The advantages and disadvantages of the present approach were critically discussed.
MATERIALS
Apparatus. A Hitachi atomic absorption spectrometer Model 170-50 with DZ lamp background compensation circuit was employed. The carbon rod was of a boat type, 2(width) x 12(length) x 5 mm(depth) of a Japan-Jarrel-Ash Model FLA 100.
Activation analyses were also used for cross-checking of the data. For this purpose, nuclear reactors, TRIGA MARK II at Rikkyo University and JRR 2-4 at Tokai Atomic Energy Institute were used along with a pulse height analyzer of Camberra Industrial Company.