𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Direct Atmospheric Pressure Coupling of Polyacrylamide Gel Electrophoresis to Mass Spectrometry for Rapid Protein Sequence Analysis

✍ Scribed by Coon, Joshua J.; Steele, Heather A.; Laipis, Philip J.; Harrison, W. W.

Book ID
126828167
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
174 KB
Volume
2
Category
Article
ISSN
1535-3893

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Laser desorption-atmospheric pressure ch
Laser desorption-atmospheric pressure chemical ionization: a novel ion source for the direct coupling of polyacrylamide gel electrophoresis to mass spectrometry
✍ Joshua J. Coon; Heather A. Steele; Philip J. Laipis; W. W. Harrison 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 161 KB

## Abstract Laser desorption‐atmospheric pressure chemical ionization‐mass spectrometry (LD‐APCI‐MS) is presented for the atmospheric pressure (AP) sampling of tryptic peptides directly from a polyacrylamide gel. In contrast to other gel sampling mass spectrometric approaches, this technique does n

Capillary electrophoresis/tandem mass sp
Capillary electrophoresis/tandem mass spectrometry for analysis of proteins from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis
✍ Xiaoying Jin; Yajuan Chen; David M. Lubman; David Misek; Samir M. Hanash 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 102 KB 👁 2 views

Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip

Detection of metals in proteins by means
Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation-inductively coupled plasma-mass spectrometry: Application to selenium
✍ Cyrille C. Chéry; Detlef Günther; Rita Cornelis; Frank Vanhaecke; Luc Moens 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 160 KB

## Abstract The capabilities of laser ablation‐inductively coupled plasma‐mass spectrometry for the detection of trace elements in a gel after gel electrophoresis were systematically studied. Figures of merit, such as limit of detection, linearity, and repeatability, were evaluated for various elem

On-line liquid chromatography/electrospr
On-line liquid chromatography/electrospray tandem mass spectrometry to investigate acrylamide adducts with cysteine residues: implications for polyacrylamide gel electrophoresis separations of proteins
✍ Marco Garzotti; Luca Rovatti; Mahmoud Hamdan 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 169 KB 👁 2 views

Adduction between acrylamide and cysteine residues is a post-translational modification associated with proteins separated by gel electrophoresis. In the present article, three model peptides containing 2-4 cysteine residues were reduced with dithiothreitol, incubated with acrylamide monomers and ex