Direct antigen detection in Langmuir—Blodgett films of immunoglobulin G modified with coproporphyrin-I
✍ Scribed by Illarion V. Turko; Galina I. Lepesheva; Vadim L. Chashchin
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 409 KB
- Volume
- 265
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
A dnect optIca method based on the mteractlon of antigen with a thm film of unmunoglobuhn G (IgG) modified with a fluorescent label was developed for the fluorimetnc determmatlon of antigen-antlbody bmdmg The analytIca system uses a Langmuir-Blodgett film of coproporphyrm I-modified IgG which binds antigen and changes Its fluorescent properties The enhanced fluorescence IS linear with respect to mcreasmg concentration of antigen over a broad range The mam advantage of the proposed method 1s the posslbdlty of developing a direct immunoassay based on evanescent excited fibre-optlcs