Direct and sequential switching from μ to ε in patients with Schistosoma mansoni infection and atopic dermatitis
✍ Scribed by Berivan Baskin; Khalid B. Islam; Birgitta Evengård; Lennart Emtestam; C. I. Edvard Smith
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 621 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
Direct and sequential switching from p to E in patients with Schistosoma mansoni infection and atopic dermatitis
Immunoglobulin isotype switching to IgE in patients infected with Schistosorna rnansoni and patients with atopic dermatitis was studied. Patients with parasitic infections or allergic diseases have a higher production of IgE. We found a fourfold increased production of IE RNA in both patient groups as compared to control donors. The increased expression of germ-line transcripts correlates with higher serum IgE levels. Nested primer polymerase chain reaction was used to generate SpISE fragments from DNA of patient peripheral blood mononuclear cells. Twenty-nine out of fourty sequenced switch fragments had undergone direct joining from Sp to SE whereas seven fragments showed mono sequential switching from Sy via either Sp, Sy2, Sy4 o r SE to SE and four fragments demonstrated double sequential switch: SpfSplSylISE, S ~~S ~~I S E I S E o r SplSyIi Sy21S~. The sequential switching had occurred either via deletions or inversions. Mapping of the breakpoints showed hot spots for recombination within Sp, Syl and SE. To our knowledge, this is the first in vivo study in humans demonstrating that switching to IgE can occur from sequential rearrangements via yl, y2 or y4.