Direct analysis of solute self-association by sedimentation equilibrium

✍ Scribed by Peter R. Wills; Michael P. Jacobsen; Donald J. Winzor

• Publisher: Wiley (John Wiley & Sons)
• Year: 1996
• Tongue: English
• Weight: 918 KB
• Volume: 38
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/(sici)1097-0282(199601)38:1<119::aid-bip10>3.0.co;2-c

✦ Synopsis

A n improved procedure is described f i r the characterization ofsoliite self-association by sedimentation equilibrium. FV'hcreas previous statistical-mechanical approaches to ullowance,fbr rhc e f f t r t s of' thermodynamic nonidealiry have entailed tedious iteration because oftheir speci-. fication of activity co<fficicnts in terms of the equilibriiim concentrations ojall species, such reliance iipon knouledge ofthe solution composition is avoided by the adaptation ofan alternative .stuti.stical-meckanical~fi,rmulation (T. L. Hill and Y. D. Chen ( I 973) Biopolymers, Vol. 12, pp. 1285-1312/ in which thermodynumic nonideality is expressed in terms of total solute concentration. The development of' an analysis in terms of'a relationship with total solute concentration as the e.-perimental variable allouv this attribute qf'the Adams-Fujita approach to be retained itithour .sacrifice c~f'.rtati.stical-inechanical rigor. Its use is illustrated by application to Rayleigh inter$winetric. record.s ofsedimentation equilibrium distributions reflecting a-chymotrjpsin dimerixtion and lysozyme self-association.