Direct analysis of mushroom constituents by mass spectrometry

✍ Scribed by G.A. McClusky; R.G. Cooks; A.M. Knevel

• Publisher: Elsevier Science
• Year: 1978
• Tongue: French
• Weight: 226 KB
• Volume: 19
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(01)95255-4

✦ Synopsis

New mass spectrometric methodology' for structural elucidation of components present in complex mixtures is here applied to a species of mushroom, Helvella esculenta. This approach is shown to have the following characteristics which are important in natural products chemistry: (i) no preparation of the biological material is required, (ii) extremely small samples suffice, (iii) highly specific structure-related information is obtained for each natural product CH $H=N /CH3 N CH$H=N N /CH3 'CH2NH2 'CH20H respectively. Since the geminal aminohydrazine, 3_, and the hydroxyhydrazine, 5, are not IJO. a6 typically stable, they probably correspond to the gas phase reaction of N-methylacetaldehydrazone with formaldehyde or methylimine. It is less likely that Kwas derived by pyrolysis or a gas phase reaction. This preliminary result raises interesting questions regarding the biogenesis of azomethine technique in studying Acknowledgment: References -p canpounds and provides further evidence of the usefulness of the MIKES natural products.