Direct analysis of microbial extracts containing metabolites of ethylenediamine-type antihistamines via high-performance liquid chromatography-thermospray mass spectrometry

✍ Scribed by W.A. Korfmacher; T.A. Getek; E.B. Hansen Jr.; C.E. Cerniglia

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 734 KB
• Volume: 185
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(90)90268-e

✦ Synopsis

The utility of high-performance liquid chromatography-thermospray mass spectrometry (HPLC-TSMS) for the characterization of the ethylenediamine-type antihistamines, pyrilamine, methapyrilene, tripelennamine, and thenyldiamine, and their methylene chloride-extractable microbial metabolites from a biological matrix is demonstrated. Typically, the [M + H]+ ion was detected as the base peak in the TS mass spectra of these compounds. The ethylenediamine-type antihistamine metabolites were detected in an extract of a fungal culture grown in the presence of 5 mg of the antihistamine. A detection limit of 200 ng was observed for the HPLC-TSMS analysis of pyrilamine.