𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Direct analysis of alkaloid profiling in plant tissue by using matrix-assisted laser desorption/ionization mass spectrometry

✍ Scribed by Wei Wu; Zhitao Liang; Zhongzhen Zhao; Zongwei Cai

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
376 KB
Volume
42
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A method for the direct determination of alkaloid profiling in plant tissues by using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) was developed. The alkaloid profiles of the herbs were obtained without the need for complicated sample preparation. Experimental results demonstrated that the direct MALDI‐TOFMS analysis allowed rapid and reliable characterization of the components in plant tissues. Four commonly used Chinese medicinal herbs were studied, including Aconitum Carmichaeli Debx. (Fuzi in Chinese) and Processed Fuzi, for herb differentiation and explanation of the significant difference in their toxicities. The direct analysis method proved valuable for the preliminary study of plant component profiles. The rapid collection of information from the direct analysis on plant tissues could be valuable for supporting the discovery of new compounds and for the quality control of medicinal herbs. Copyright © 2006 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Direct analysis of drug candidates in ti
Direct analysis of drug candidates in tissue by matrix-assisted laser desorption/ionization mass spectrometry
✍ Michelle L. Reyzer; Yunsheng Hsieh; Kwokei Ng; Walter A. Korfmacher; Richard M. 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 409 KB

## Abstract Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) has been used to directly analyze and image pharmaceutical compounds in intact tissue. The anti‐tumor drug SCH 226374 was unambiguously determined in mouse tumor tissue using MALDI‐QqTOFMS (QSTAR) by monitoring the

Direct peptide profiling of single neuro
Direct peptide profiling of single neurons by matrix-assisted laser desorption–ionization mass spectrometry
✍ P. A. van Veelen; C. R. Jiménez; K. W. Li; W. C. Wildering; W. P. M. Geraerts; U 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 451 KB

Peptide profiles of single neurons in Lymnaea stagnalis were directly characterized by matrix-assisted laser desorptiowionization mass spectrometry. The mass analysis was performed after minor pretreatment and without any separation steps. Good-quality spectra were obtained of several cell types and

Quantitative Analysis of Oligonucleotide
Quantitative Analysis of Oligonucleotides by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
✍ Bernd A. Bruenner; Tai-Tung Yip; T. William Hutchens 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 434 KB 👁 2 views

Quantitative aspects of oligonucleotide analysis by matrix-assisted laser desorption/iotion (MALDI) mass spectrometry remain largely unexplored relative to the efforts that have been devoted to quantitative peptide and protein analysis. The successful quantitation of these other biopolymers coupled

Glycopeptide profiling of human urinary
Glycopeptide profiling of human urinary erythropoietin by matrix-assisted laser desorption/ionization mass spectrometry
✍ Rahbek-Nielsen, Henrik; Roepstorff, Peter; Reischl, Heinz; Wozny, Manfred; Koll, 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 597 KB

The site-speciÐc glycan heterogeneity of human urinary erythropoietin was investigated by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Owing to the small amount of protein available, a strategy combining optimal sensitivity and speciÐcity was used. Erythropoietin was red

Characterization of plant oligosaccharid
Characterization of plant oligosaccharides by matrix-assisted laser desorption/ionization and electrospray mass spectrometry
✍ Lobvi E. Matamoros Fernández; Nicolai Obel; Henrik Vibe Scheller; Peter Roepstor 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 262 KB 👁 1 views

## Abstract Structural characterization of arabinoxylans from wheat by matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) and electrospray ionization (ESI) mass spectrometry using a Q‐TOF mass analyser (ESI‐Q‐TOF) or an ion trap (IT) mass analyser is presented. An arabinoxylan s